Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Myosin Va transport of liposomes in three-dimensional actin networks is modulated by actin filament density, position, and polarity

doi: 10.1073/pnas.1901176116

Figure Lengend Snippet: Myosin Va teams transport intracellular cargo through networks of actin. (A) Lipid-bound cargo is produced and packaged at the interior of the cell within the Golgi. These cargos are first transported along microtubule tracks, followed by handoff to MyoVa for distribution and final delivery to sites of secretion at the cell membrane. (B) Superresolution, 3D STORM reconstruction of an in vitro actin network. Actin filaments are strung between silica beads of varying diameters, which support the network and maintain a 3D organization. Color represents z position. (Scale bar: 2 µm.) (C) Overlay of 350-nm vesicle trajectory (magenta) by teams of MyoVa within a 3D actin filament network (colored by z position). (Scale bar: 1 µm.)

Article Snippet: 3D STORM images were acquired using a Nikon N-STORM superresolution microscope system with excitation of Alexa-647 phalloidin-labeled actin by 647- and 405-nm lasers.

Techniques: Produced, In Vitro